Publications

Litzau L, Wren E, Kruse A, Blacklow SC. Structural basis for tetraspanin-dependent surface export and adhesive function of integrin α3β1. bioRxiv [Preprint]. 2026 September 3:2026.09.01.748654: doi: 10.64898/2026.09.01.748654.

Ferreira AP, Wang S, Poluben L, Morais D, Brandstadter J, Perkey E, Sotirakos S, Drew M, Pan L, Lemieux ME, Fernandes SM, Dorfman DM, Shoji B, Stowell SR, Maillard I, Brown J, Blacklow SC, Aster JC. CCL19⁺ fibroblasts define a proliferative niche in chronic lymphocytic leukemia. bioRxiv [Preprint]. 2026 May 27:2024.11.15.623868. doi: 10.1101/2024.11.15.623868. PMID: 42244527; PMCID: PMC13232244.

Mout R, Jing R, Tanaka-Yano M, Egan ED, Eisenach H, Kononov MA, Windisch R, Najia MAT, Tompkins A, Hensch L, Bingham T, Gunage R, Zhao Y, Edman NI, Li C, Wang D, Schlaeger TM, Zon LI, North TE, Lendahl U, Rowe RG, Baker D, Blacklow SC, Daley GQ. Design of soluble Notch agonists that drive T cell development and boost immunity. Cell. 2025 Oct 16;188(21):5980-5994.e28. doi: 10.1016/j.cell.2025.07.009. Epub 2025 Aug 1. PMID: 40752493; PMCID: PMC12327808.

Cao R, Jones DTD, Pan L, Yang A, Wang S, Padi SKR, Rawson S, Aster JC, Blacklow SC. Molecular mechanism of PP2A/B55α phosphatase inhibition by IER5. Cell Chem Biol. 2025 Apr 17;32(4):631-642.e7. doi: 10.1016/j.chembiol.2025.03.004. Epub 2025 Apr 9. PMID: 40209703.

Cao R, Gozlan O, Airich A, Tveriakhina L, Zhou H, Jiang H, Cole PA, Aster JC, Klein T, Sprinzak D, Blacklow SC. Structural requirements for activity of Mind bomb1 in Notch signaling. Structure. 2024 Oct 3;32(10):1667-1676.e5. doi: 10.1016/j.str.2024.07.011. Epub 2024 Aug 8.PMID: 39121852.

Dates AN, Jones DTD, Smith JS, Skiba MA, Rich MF, Burruss MM, Kruse AC, Blacklow SC. Heterogeneity of tethered agonist signaling in adhesion G protein-coupled receptors. Cell Chem Biol. 2024 Aug 15;31(8):1542-1553.e4. doi: 10.1016/j.chembiol.2024.03.004. Epub 2024.

Tveriakhina L, Scanavachi G, Egan ED, Da Cunha Correia RB, Martin AP, Rogers JM, Yodh JS, Aster JC, Kirchhausen T, Blacklow SC. Temporal dynamics and stoichiometry in human Notch signaling from Notch synaptic complex formation to nuclear entry of the Notch intracellular domain. Dev Cell. 2024 Jun 3;59(11):1425-1438.e8. doi: 10.1016/j.devcel.2024.03.021. Epub 2024 Apr 3.PMID: 38574735.

Martin AP, Bradshaw GA, Eisert RJ, Egan ED, Tveriakhina L, Rogers JM, Dates AN, Scanavachi G, Aster JC, Kirchhausen T, Kalocsay M, Blacklow SC. A spatiotemporal Notch interaction map from plasma membrane to nucleus. Sci Signal. 2023 Aug;16 (796):eadg6474. doi: 10.1126/scisignal.adg6474. Epub 2023 Aug 1. PMID: 37527352.

Lipper CH, Egan ED, Gabriel KH, Blacklow SC. Structural basis for membrane-proximal proteolysis of substrates by ADAM10. Cell  2023 Aug 17;186(17):3632-3641.e10. doi: 10.1016/j.cell.2023.06.026. Epub 2023 Jul 28. PMID: 37516108.

Jones DTD, Dates AN, Rawson SD, Burruss MN, Lipper CH, Blacklow SC. Tethered agonist activated ADGRF1 structure and signalling analysis reveal basis for G protein coupling. Nature Communications 2023 Apr 29;14(1):2490. doi: 10.1038/s41467-023-38083-7

Lipper CH, Gabriel K, Seegar TC, Dürr KL, Tomlinson MG, Blacklow SC. Crystal structure of the Tspan15 LEL domain reveals a conserved ADAM10 binding site. Structure 2022 Feb 3;30(2):206-214. doi: 10.1016/j.str.2021.10.007

Vemulapalli V, Donovan KA, Seegar TC, Rogers JM, Bae M, Lumpkin RJ, Cao R, Henke MT, Ray SS, Fischer ES, Cuny GD, Blacklow SC. Targeted Degradation of the Oncogenic Phosphatase SHP2. ACS Chem Bio. 2021 Aug 19;60(34):2593-2609. doi: 10.1021/acs.biochem.1c00377.

Vemulapalli V, Chylek LA, Erickson A, Pfeiffer A, Gabriel K, LaRochelle J, Subramanian K, Cao R, Stegmaier K, Mohseni M, LaMarche MJ, Acker MG, Sorger PK, Gygi SP, Blacklow SC. Time-resolved phosphoproteomics reveals scaffolding and catalysis-responsive patterns of SHP2-dependent signaling. eLife 2021 Mar 23;10. pii: e64251 doi: 10.7554/eLife.64251.

Susa KJ, Rawson S, Kruse AC, Blacklow SC. Cryo-EM structure of the B cell co-receptor CD19 bound to the tetraspanin CD81. Science. 2021 Jan 15;371(6526):300-305. doi: 10.1126/science.abd9836.

Susa KJ, Seegar TCM, Blacklow SC, Kruse AC. A dynamic interaction between CD19 and the tetraspanin CD81 controls B cell co-receptor trafficking. Elife. 2020 Apr 27;9. pii: e52337. doi: 10.7554/eLife.52337.

Rogers JM, Guo B, Egan ED, Aster JC, Adelman K, Blacklow SC. MAML1-dependent Notch responsive genes exhibit differing co-factor requirements for transcriptional activation. Mol Cell Biol. 2020 Mar 16. pii: MCB.00014-20. doi: 10.1128/MCB.00014-20.

Rome KS, Stein SJ, Kurachi M, Petrovic J, Schwartz GW, Mack EA, Uljon S, Wu WW, DeHart AG, McClory SE, Xu L, Gimotty PA, Blacklow SC, Faryabi RB, Wherry EJ, Jordan MS, Pear WS.Trib1 regulates T cell differentiation during chronic infection by restraining the effector program. J Exp Med. 2020 May 4;217(5). pii: e20190888. doi: 10.1084/jem.20190888.

Adhikari AA, Seegar TCM, Ficarro SB, McCurry MD, Ramachandran D, Yao L, Chaudhari SN, Ndousse-Fetter S, Banks AS, Marto JA, Blacklow SC, Devlin AS. Development of a covalent inhibitor of gut bacterial bile salt hydrolases. Nat Chem Biol. 2020 Mar;16(3):318-326. doi: 10.1038/s41589-020-0467-3.

Jarrett SM, Seegar TCM, Andrews M, Adelmant G, Marto JA, Aster JC, Blacklow SC. Extension of the Notch intracellular domain ankyrin repeat stack by NRARP promotes feedback inhibition of Notch signaling. Sci Signal. 2019 Nov 5;12(606). pii: eaay2369. doi: 10.1126/scisignal.aay2369.

Seegar TC, Blacklow SC. Domain integration of ADAM family proteins: Emerging themes from structural studies. Exp Biol Med (Maywood). 2019 Dec;244(17):1510-1519. doi: 10.1177/1535370219865901.

Drabek AA, Loparo JJ, Blacklow SC. A Flow-Extension Tethered Particle Motion Assay for Single-Molecule Proteolysis. Biochemistry. 2019 May 21;58(20):2509-2518. doi: 10.1021/acs.biochem.9b00106.

Rogers JM, Waters CT, Seegar TCM, Jarrett SM, Hallworth AN, Blacklow SC, Bulyk ML. Bispecific Forkhead Transcription Factor FoxN3 Recognizes Two Distinct Motifs with Different DNA Shapes. Mol Cell. 2019 Apr 18;74(2):245-253.e6. doi: 10.1016/j.molcel.2019.01.019.

Petrovic J, Zhou Y, Fasolino M, Goldman N, Schwartz GW, Mumbach MR, Nguyen SC, Rome KS, Sela Y, Zapataro Z, Blacklow SC, Kruhlak MJ, Shi J, Aster JC, Joyce EF, Little SC, Vahedi G, Pear WS, Faryabi RB. Oncogenic Notch Promotes Long-Range Regulatory Interactions within Hyperconnected 3D Cliques. Mol Cell. 2019 Mar 21;73(6):1174-1190.e12. doi: 10.1016/j.molcel.2019.01.006.

LaRochelle JR, Fodor M, Vemulapalli V, Mohseni M, Wang P, Stams T, LaMarche MJ, Chopra R, Acker MG, Blacklow SC. Structural reorganization of SHP2 by oncogenic mutations and implications for oncoprotein resistance to allosteric inhibition. Nat Commun. 2018 Oct 30;9(1):4508. doi: 10.1038/s41467-018-06823-9.

Tveriakhina L, Schuster-Gossler K, Jarrett SM, Andrawes MB, Rohrbach M, Blacklow SC, Gossler A. The ectodomains determine ligand function in vivo and selectivity of DLL1 and DLL4 toward NOTCH1 and NOTCH2 in vitro. Elife. 2018 Oct 5;7. pii: e40045. doi: 10.7554/eLife.40045.

Wang J, Erazo T, Ferguson FM, Buckley DL, Gomez N, Muñoz-Guardiola P, Diéguez-Martínez N, Deng X, Hao M, Massefski W, Fedorov O, Offei-Addo NK, Park PM, Dai L, DiBona A, Becht K, Kim ND, McKeown MR, Roberts JM, Zhang J, Sim T, Alessi DR, Bradner JE, Lizcano JM, Blacklow SC, Qi J, Xu X, Gray NS. Structural and Atropisomeric Factors Governing the Selectivity of Pyrimido-benzodiazipinones as Inhibitors of Kinases and Bromodomains. ACS Chem Biol. 2018 Sep 21;13(9):2438-2448. doi: 10.1021/acschembio.7b00638.

Lovendahl KN, Blacklow SC, Gordon WR. The Molecular Mechanism of Notch Activation. Adv Exp Med Biol. 2018;1066:47-58. doi: 10.1007/978-3-319-89512-3_3.

Fodor M, Price E, Wang P, Lu H, Argintaru A, Chen Z, Glick M, Hao HX, Kato M, Koenig R, LaRochelle JR, Liu G, McNeill E, Majumdar D, Nishiguchi GA, Perez LB, Paris G, Quinn CM, Ramsey T, Sendzik M, Shultz MD, Williams SL, Stams T, Blacklow SC, Acker MG, LaMarche MJ. Dual Allosteric Inhibition of SHP2 Phosphatase. ACS Chem Biol. 2018;13(3):647-656. doi: 10.1021/acschembio.7b00980. PMID: 29304282.

LaRochelle JR, Fodor M, Ellegast JM, Liu X, Vemulapalli V, Mohseni M, Stams T, Buhrlage SJ, Stegmaier K, LaMarche MJ, Acker MG, Blacklow SC. Identification of an allosteric benzothiazolopyrimidone inhibitor of the oncogenic protein tyrosine phosphatase SHP2. Bioorg Med Chem. 2017;25(24):6479-6485. doi: 10.1016/j.bmc.2017.10.025. PMID: 29089257.

Seegar, TCM, Killingsworth, LB, Saha, N, Meyer, PA, Patra, D, Zimmerman, B, Janes, PW, Rubinstein, E, Nikolov, DB, Skiniotis, G, Kruse, AC, and Blacklow SC. Structural Basis for Regulated Proteolysis by the α-Secretase ADAM10. Cell 2017;171:1638-1648.e7. PMID: 29224781. PMCID: PMC5773094.

Pajcini KV, Xu L, Shao L, Petrovic J, Palasiewicz K, Ohtani Y, Bailis W, Lee C, Wertheim GB, Mani R, Musuthamy N, Li Y, Meijerink JPP, Blacklow SC, Faryabi RB, Cherry S, Pear WS. MAFB enhances oncogenic Notch signaling in T cell acute lymphoblastic leukemia. Sci Signal. 2017;10(505). pii: eaam6846. doi: 10.1126/scisignal.aam6846. PMID: 29138297. PMCID: PMC5885022.

Sajed DP, Faquin WC, Carey C, Severson EA, Afrogheh AH, Johnson CA, Blacklow SC, Chau NG, Lin DT, Krane JF, Jo VY, Garcia JJ, Sholl LM, Aster JC. Diffuse Staining for Activated NOTCH1 Correlates With NOTCH1 Mutation Status and Is Associated With Worse Outcome in Adenoid Cystic Carcinoma. Am J Surg Pathol. 2017 Nov;41(11):1473-1482. doi: 10.1097/PAS.0000000000000945. PMID: 28914715. PMCID: PMC5657508.

Ryan RJH, Petrovic J, Rausch DM, Zhou Y, Lareau CA, Kluk MJ, Christie AL, Lee WY, Tarjan DR, Guo B, Donohue LKH, Gillespie SM, Nardi V, Hochberg EP, Blacklow SC, Weinstock DM, Faryabi RB, Bernstein BE, Aster JC, Pear WS. A B Cell Regulome Links Notch to Downstream Oncogenic Pathways in Small B Cell Lymphomas. Cell Rep. 2017;21(3):784-797. doi: 10.1016/j.celrep.2017.09.066. PMID: 29045844. PMCID: PMC5687286.

Severson E, Arnett K, Wang H, Zang C, Liu H, Pear WS, Liu X, Blacklow SC*, and Aster JC*. Genome-wide identification and characterization of Notch transcription complex-binding sequence paired sites in leukemia cells. Science Signaling 2017;10:477. doi: 10.1126/scisignal.aag1598. PMID: 28465412.

*Co-corresponding authors.

McMillan BJ, Tibbe C, Drabek AA, Seegar TCM, Blacklow SC*, and Klein T*. Structural Basis for Regulation of ESCRT-III Complexes by Lgd. Cell Rep. 2017 May 30;19(9):1750-1757. doi: 10.1016/j.celrep.2017.05.026. PMID: 28564595. PMCID: PMC5528166.

*Co-corresponding authors.

Choi SH, Severson E, Pear WS, Liu XS, Aster JC*, Blacklow SC*. The common oncogenomic program of NOTCH1 and NOTCH3 signaling in T-cell acute lymphoblastic leukemia. PLoS One 2017;12(10):e0185762. doi: 10.1371/journal.pone.0185762. PMID: 29023469. PMC5638296.

McMillan BJ, Zimmerman B, Egan ED, Lofgren, M, Xu X, Hesser A, and Blacklow SC. Structure of human POFUT1, its requirement in ligand-independent oncogenic notch signaling, and functional effects of Dowling-Degos mutations. Glycobiology, 2017, 27(8):777-786. doi: 10.1093/glycob/cwx020. PMID: 28334865. PMC5881682.

Durzynska I, Xu X, Adelmant G, Ficarro SB, Marto JA, Sliz P, Uljon S, and Blacklow SC. STK40 Is a Pseudokinase that Binds the E3 Ubiquitin Ligase COP1. Structure 2017:25, 287-294. doi: 10.1016/j.str.2016.12.008. PMID: 28089446. PMCID: PMC5299031.

Zimmerman B, Kelly B, McMillan, BJ, Seegar, TCM, Dror, RO, Kruse, AC, and Blacklow SC. Crystal Structure of a Full-Length Human Tetraspanin Reveals a Cholesterol-Binding Pocket. Cell 2016;167:1041-1051. doi: 10.1016/j.cell.2016.09.056. PMCID: PMC5127602.

McMillan BJ, Tibbe C, Jeon H, Drabek AA, Klein T and Blacklow SC. Electrostatic Interactions between Elongated Monomers Drive Filamentation of Drosophila Shrub, a Metazoan ESCRT-III Protein. Cell Reports 2016;16(5):1211-1217. doi: 10.1016/j.celrep.2016.06.093. PMCID: PMC4985235.

Bernasconi-Elias P, Hu T, Jenkins D, Firestone B, Gans S, Kurth E, Capodieci P, Deplazes-Lauber J, Petropoulos K, Thiel P, Ponsel D, Hee Choi S, LeMotte P, London A, Goetcshkes M, Nolin E, Jones MD, Slocum K, Kluk MJ, Weinstock DM, Christodoulou A, Weinberg O, Jaehrling J, Ettenberg SA, Buckler A, Blacklow SC, Aster JC, Fryer CJ. Characterization of activating mutations of NOTCH3 in T-cell acute lymphoblastic leukemia and anti-leukemic activity of NOTCH3 inhibitory antibodies. Oncogene. 2016 May 9. doi: 10.1038/onc.2016.133. PMID: 27157619. PMCID: PMC5102827.

LaRochelle JR, Fodor M, Xu X, Durzynska I, Fan L, Stams T, Chan HM, LaMarche MJ, Chopra R, Wang P, Fortin PD, Acker MG, Blacklow SC. Structural and Functional Consequences of Three Cancer-Associated Mutations of the Oncogenic Phosphatase SHP2. Biochemistry 2016;55(15):2269-2277. doi: 10.1021/acs.biochem.5b01287. PMID: 27030275. PMCID: PMC4900891.

Uljon S, Xu X, Durzynska I, Stein S, Adelmant G, Marto JA, Pear WS, Blacklow SC. Structural Basis for Substrate Selectivity of the E3 Ligase COP1. Structure. 2016;24(5):687-96. doi: 10.1016/j.str.2016.03.002. PMID: 27041596. PMCID: PMC4856590

Meyer PA, Socias S, Key J, Ransey E, Tjon EC, Buschiazzo A, Lei M, Botka C, Withrow J, Neau D, Rajashankar K, Anderson KS, Baxter RH, Blacklow SC, Boggon TJ, Bonvin AM, Borek D, Brett TJ, Caflisch A, Chang CI, Chazin WJ, Corbett KD, Cosgrove MS, Crosson S, Dhe-Paganon S, Di Cera E, Drennan CL, Eck MJ, Eichman BF, Fan QR, Ferré-D'Amaré AR, Christopher Fromme J, Garcia KC, Gaudet R, Gong P, Harrison SC, Heldwein EE, Jia Z, Keenan RJ, Kruse AC, Kvansakul M, McLellan JS, Modis Y, Nam Y, Otwinowski Z, Pai EF, Pereira PJ, Petosa C, Raman CS, Rapoport TA, Roll-Mecak A, Rosen MK, Rudenko G, Schlessinger J, Schwartz TU, Shamoo Y, Sondermann H, Tao YJ, Tolia NH, Tsodikov OV, Westover KD, Wu H, Foster I, Fraser JS, Maia FR, Gonen T, Kirchhausen T, Diederichs K, Crosas M, Sliz P. Data publication with the structural biology data grid supports live analysis. Nat Commun. 2016;7:10882. doi: 10.1038/ncomms10882. PMID: 26947396. PMCID: PMC4786681.

Knoechel B, Bhatt A, Pan L, Pedamallu CS, Severson E, Gutierrez A, Dorfman DM, Kuo FC, Kluk M, Kung AL, Zweidler-McKay P, Meyerson M, Blacklow SC, DeAngelo DJ, Aster JC. Complete hematologic response of early T-cell progenitor acute lymphoblastic leukemia to the γ-secretase inhibitor BMS-906024: genetic and epigenetic findings in an outlier case. Cold Spring Harb Mol Case Stud. 2015;1(1):a000539. doi: 10.1101/mcs.a000539. PMID: 27148573. PMCID: PMC4850884.

Xu X, Choi SH, Hu T, Tiyanont K, Habets R, Groot AJ, Vooijs M, Aster JC, Chopra R, Fryer C, Blacklow SC. Insights into Autoregulation of Notch3 from Structural and Functional Studies of Its Negative Regulatory Region. Structure. 2015 Jul 7;23(7):1227-35. doi: 10.1016/j.str.2015.05.001. Epub 2015 Jun 4. PMID: 26051713; PMCID: PMC4497832.

Gordon WR, Zimmerman B, He L, Miles LJ, Huang J, Tiyanont K, McArthur DG, Aster JC, Perrimon N, Loparo JJ, Blacklow SC. Mechanical Allostery: Evidence for a Force Requirement in the Proteolytic Activation of Notch. Dev Cell. 2015 Jun 22;33(6):729-36. doi: 10.1016/j.devcel.2015.05.004. Epub 2015 Jun 4. PMID: 26051539; PMCID: PMC4481192.

Habets RA, Groot AJ, Yahyanejad S, Tiyanont K, Blacklow SC, Vooijs M. Human NOTCH2 Is Resistant to Ligand-independent Activation by Metalloprotease Adam17. J Biol Chem. 2015 Jun 5;290(23):14705-16. doi: 10.1074/jbc.M115.643676. Epub 2015 Apr 27. PMID: 25918160; PMCID: PMC4505536.

McMillan BJ, Schnute B, Ohlenhard N, Zimmerman B, Miles L, Beglova N, Klein T, Blacklow SC. A tail of two sites: a bipartite mechanism for recognition of notch ligands by mind bomb E3 ligases. Mol Cell. 2015 Mar 5;57(5):912-924. doi: 10.1016/j.molcel.2015.01.019. Erratum in: Mol Cell. 2015 Apr 16;58(2):387. PMID: 25747658; PMCID: PMC4355479.

Yashiro-Ohtani Y, Wang H, Zang C, Arnett KL, Bailis W, Ho Y, Knoechel B, Lanauze C, Louis L, Forsyth KS, Chen S, Chung Y, Schug J, Blobel GA, Liebhaber SA, Bernstein BE, Blacklow SC, Liu XS, Aster JC, Pear WS. Long-range enhancer activity determines Myc sensitivity to Notch inhibitors in T cell leukemia. Proc Natl Acad Sci U S A. 2014 Nov 18;111(46):E4946-53. doi: 10.1073/pnas.1407079111. Epub 2014 Nov 4. PMID: 25369933; PMCID: PMC4246292.

McKeown MR, Shaw DL, Fu H, Liu S, Xu X, Marineau JJ, Huang Y, Zhang X, Buckley DL, Kadam A, Zhang Z, Blacklow SC, Qi J, Zhang W, Bradner JE. Biased multicomponent reactions to develop novel bromodomain inhibitors. J Med Chem. 2014 Nov 13;57(21):9019-27. doi: 10.1021/jm501120z. Epub 2014 Oct 31. PMID: 25314271; PMCID: PMC4234447.

Arnett KL, Blacklow SC. Analyzing the nuclear complexes of Notch signaling by electrophoretic mobility shift assay. Methods Mol Biol. 2014;1187:231-45. doi: 10.1007/978-1-4939-1139-4_18. PMID: 25053494; PMCID: PMC4410701.

Wang H, Zang C, Taing L, Arnett KL, Wong YJ, Pear WS, Blacklow SC, Liu XS, Aster JC. NOTCH1-RBPJ complexes drive target gene expression through dynamic interactions with superenhancers. Proc Natl Acad Sci U S A. 2014 Jan 14;111(2):705-10. doi: 10.1073/pnas.1315023111. Epub 2013 Dec 27. PMID: 24374627; PMCID: PMC3896193.

Blacklow SC. Refining a Jagged edge. Structure. 2013 Dec 3;21(12):2100-1. doi: 10.1016/j.str.2013.11.001. PMID: 24315457; PMCID: PMC3892149.

Tiyanont K, Wales TE, Siebel CW, Engen JR, Blacklow SC. Insights into Notch3 activation and inhibition mediated by antibodies directed against its negative regulatory region. J Mol Biol. 2013 Sep 9;425(17):3192-204. doi: 10.1016/j.jmb.2013.05.025. Epub 2013 Jun 6. PMID: 23747483; PMCID: PMC3751422.

Andrawes MB, Xu X, Liu H, Ficarro SB, Marto JA, Aster JC, Blacklow SC. Intrinsic selectivity of Notch 1 for Delta-like 4 over Delta-like 1. J Biol Chem. 2013 Aug 30;288(35):25477-25489. doi: 10.1074/jbc.M113.454850. Epub 2013 Jul 9. PMID: 23839946; PMCID: PMC3757209.

Roti G, Carlton A, Ross KN, Markstein M, Pajcini K, Su AH, Perrimon N, Pear WS, Kung AL, Blacklow SC, Aster JC, Stegmaier K. Complementary genomic screens identify SERCA as a therapeutic target in NOTCH1 mutated cancer. Cancer Cell. 2013 Mar 18;23(3):390-405. doi: 10.1016/j.ccr.2013.01.015. Epub 2013 Feb 21. PMID: 23434461; PMCID: PMC3709972.

Aster JC, Blacklow SC. Targeting the Notch pathway: twists and turns on the road to rational therapeutics. J Clin Oncol. 2012 Jul 1;30(19):2418-20. doi: 10.1200/JCO.2012.42.0992. Epub 2012 May 14. PMID: 22585704.

O'Donovan DJ, Stokes-Rees I, Nam Y, Blacklow SC, Schröder GF, Brunger AT, Sliz P. A grid-enabled web service for low-resolution crystal structure refinement. Acta Crystallogr D Biol Crystallogr. 2012 Mar;68(Pt 3):261-7. doi: 10.1107/S0907444912001163. Epub 2012 Feb 14. PMID: 22349228; PMCID: PMC3282622.

Choi SH, Wales TE, Nam Y, O'Donovan DJ, Sliz P, Engen JR, Blacklow SC. Conformational locking upon cooperative assembly of notch transcription complexes. Structure. 2012 Feb 8;20(2):340-9. doi: 10.1016/j.str.2011.12.011. PMID: 22325781; PMCID: PMC3285698.

Germar K, Dose M, Konstantinou T, Zhang J, Wang H, Lobry C, Arnett KL, Blacklow SC, Aifantis I, Aster JC, Gounari F. T-cell factor 1 is a gatekeeper for T-cell specification in response to Notch signaling. Proc Natl Acad Sci U S A. 2011 Dec 13;108(50):20060-5. doi: 10.1073/pnas.1110230108. Epub 2011 Nov 22. PMID: 22109558; PMCID: PMC3250146.

Wang NJ, Sanborn Z, Arnett KL, Bayston LJ, Liao W, Proby CM, Leigh IM, Collisson EA, Gordon PB, Jakkula L, Pennypacker S, Zou Y, Sharma M, North JP, Vemula SS, Mauro TM, Neuhaus IM, Leboit PE, Hur JS, Park K, Huh N, Kwok PY, Arron ST, Massion PP, Bale AE, Haussler D, Cleaver JE, Gray JW, Spellman PT, South AP, Aster JC, Blacklow SC, Cho RJ. Loss-of-function mutations in Notch receptors in cutaneous and lung squamous cell carcinoma. Proc Natl Acad Sci U S A. 2011 Oct 25;108(43):17761-6. doi: 10.1073/pnas.1114669108. Epub 2011 Oct 17. PMID: 22006338; PMCID: PMC3203814.

Zhao B, Zou J, Wang H, Johannsen E, Peng CW, Quackenbush J, Mar JC, Morton CC, Freedman ML, Blacklow SC, Aster JC, Bernstein BE, Kieff E. Epstein-Barr virus exploits intrinsic B-lymphocyte transcription programs to achieve immortal cell growth. Proc Natl Acad Sci U S A. 2011 Sep 6;108(36):14902-7. doi: 10.1073/pnas.1108892108. Epub 2011 Jul 11. PMID: 21746931; PMCID: PMC3169132.

Wang H, Zou J, Zhao B, Johannsen E, Ashworth T, Wong H, Pear WS, Schug J, Blacklow SC, Arnett KL, Bernstein BE, Kieff E, Aster JC. Genome-wide analysis reveals conserved and divergent features of Notch1/RBPJ binding in human and murine T-lymphoblastic leukemia cells. Proc Natl Acad Sci U S A. 2011 Sep 6;108(36):14908-13. doi: 10.1073/pnas.1109023108. Epub 2011 Jul 7. PMID: 21737748; PMCID: PMC3169118.

Calderwood MA, Lee S, Holthaus AM, Blacklow SC, Kieff E, Johannsen E. Epstein-Barr virus nuclear protein 3C binds to the N-terminal (NTD) and beta trefoil domains (BTD) of RBP/CSL; only the NTD interaction is essential for lymphoblastoid cell growth. Virology. 2011 May 25;414(1):19-25. doi: 10.1016/j.virol.2011.02.018. Epub 2011 Mar 26. PMID: 21440926; PMCID: PMC3104408.

Tiyanont K, Wales TE, Aste-Amezaga M, Aster JC, Engen JR, Blacklow SC. Evidence for increased exposure of the Notch1 metalloprotease cleavage site upon conversion to an activated conformation. Structure. 2011 Apr 13;19(4):546-54. doi: 10.1016/j.str.2011.01.016. PMID: 21481777; PMCID: PMC3075624.

Aster JC, Blacklow SC, Pear WS. Notch signalling in T-cell lymphoblastic leukaemia/lymphoma and other haematological malignancies. J Pathol. 2011 Jan;223(2):262-73. doi: 10.1002/path.2789. Epub 2010 Oct 21. PMID: 20967796; PMCID: PMC2996483.

Aster JC, Bodnar N, Xu L, Karnell F, Milholland JM, Maillard I, Histen G, Nam Y, Blacklow SC, Pear WS. Notch ankyrin repeat domain variation influences leukemogenesis and Myc transactivation. PLoS One. 2011;6(10):e25645. doi: 10.1371/journal.pone.0025645. Epub 2011 Oct 13. PMID: 22022427; PMCID: PMC3192765.

Ashworth TD, Pear WS, Chiang MY, Blacklow SC, Mastio J, Xu L, Kelliher M, Kastner P, Chan S, Aster JC. Deletion-based mechanisms of Notch1 activation in T-ALL: key roles for RAG recombinase and a conserved internal translational start site in Notch1. Blood. 2010 Dec 16;116(25):5455-64. doi: 10.1182/blood-2010-05-286328. Epub 2010 Sep 17. PMID: 20852131; PMCID: PMC3031398.

Keeshan K, Bailis W, Dedhia PH, Vega ME, Shestova O, Xu L, Toscano K, Uljon SN, Blacklow SC, Pear WS. Transformation by Tribbles homolog 2 (Trib2) requires both the Trib2 kinase domain and COP1 binding. Blood. 2010 Dec 2;116(23):4948-57. doi: 10.1182/blood-2009-10-247361. Epub 2010 Aug 30. PMID: 20805362; PMCID: PMC3012589.

Liu H, Chi AW, Arnett KL, Chiang MY, Xu L, Shestova O, Wang H, Li YM, Bhandoola A, Aster JC, Blacklow SC, Pear WS. Notch dimerization is required for leukemogenesis and T-cell development. Genes Dev. 2010 Nov 1;24(21):2395-407. doi: 10.1101/gad.1975210. Epub 2010 Oct 8. PMID: 20935071; PMCID: PMC2964750.

Arnett KL, Hass M, McArthur DG, Ilagan MX, Aster JC, Kopan R, Blacklow SC. Structural and mechanistic insights into cooperative assembly of dimeric Notch transcription complexes. Nat Struct Mol Biol. 2010 Nov;17(11):1312-7. doi: 10.1038/nsmb.1938. Epub 2010 Oct 24. PMID: 20972443; PMCID: PMC3024583.

Dedhia PH, Keeshan K, Uljon S, Xu L, Vega ME, Shestova O, Zaks-Zilberman M, Romany C, Blacklow SC, Pear WS. Differential ability of Tribbles family members to promote degradation of C/EBPalpha and induce acute myelogenous leukemia. Blood. 2010 Aug 26;116(8):1321-8. doi: 10.1182/blood-2009-07-229450. Epub 2010 Apr 21. PMID: 20410507; PMCID: PMC2938240.

Kovall RA, Blacklow SC. Mechanistic insights into Notch receptor signaling from structural and biochemical studies. Curr Top Dev Biol. 2010;92:31-71. doi: 10.1016/S0070-2153(10)92002-4. PMID: 20816392.

Aste-Amézaga M, Zhang N, Lineberger JE, Arnold BA, Toner TJ, Gu M, Huang L, Vitelli S, Vo KT, Haytko P, Zhao JZ, Baleydier F, L'Heureux S, Wang H, Gordon WR, Thoryk E, Andrawes MB, Tiyanont K, Stegmaier K, Roti G, Ross KN, Franlin LL, Wang H, Wang F, Chastain M, Bett AJ, Audoly LP, Aster JC, Blacklow SC, Huber HE. Characterization of Notch1 antibodies that inhibit signaling of both normal and mutated Notch1 receptors. PLoS One. 2010 Feb 8;5(2):e9094. doi: 10.1371/journal.pone.0009094. PMID: 20161710; PMCID: PMC2817004.

Del Bianco C, Vedenko A, Choi SH, Berger MF, Shokri L, Bulyk ML, Blacklow SC. Notch and MAML-1 complexation do not detectably alter the DNA binding specificity of the transcription factor CSL. PLoS One. 2010 Nov 24;5(11):e15034. doi: 10.1371/journal.pone.0015034. PMID: 21124806; PMCID: PMC2991368.

Moellering RE, Cornejo M, Davis TN, Del Bianco C, Aster JC, Blacklow SC, Kung AL, Gilliland DG, Verdine GL, Bradner JE. Direct inhibition of the NOTCH transcription factor complex. Nature. 2009 Nov 12;462(7270):182-8. doi: 10.1038/nature08543. Erratum in: Nature. 2010 Jan 21;463(7279):384. PMID: 19907488; PMCID: PMC2951323.

Yashiro-Ohtani Y, He Y, Ohtani T, Jones ME, Shestova O, Xu L, Fang TC, Chiang MY, Intlekofer AM, Blacklow SC, Zhuang Y, Pear WS. Pre-TCR signaling inactivates Notch1 transcription by antagonizing E2A. Genes Dev. 2009 Jul 15;23(14):1665-76. doi: 10.1101/gad.1793709. PMID: 19605688; PMCID: PMC2714710.

Gordon WR, Roy M, Vardar-Ulu D, Garfinkel M, Mansour MR, Aster JC, Blacklow SC. Structure of the Notch1-negative regulatory region: implications for normal activation and pathogenic signaling in T-ALL. Blood. 2009 Apr 30;113(18):4381-90. doi: 10.1182/blood-2008-08-174748. Epub 2008 Dec 15. PMID: 19075186; PMCID: PMC2676092.

Gordon WR, Vardar-Ulu D, L'Heureux S, Ashworth T, Malecki MJ, Sanchez-Irizarry C, McArthur DG, Histen G, Mitchell JL, Aster JC, Blacklow SC. Effects of S1 cleavage on the structure, surface export, and signaling activity of human Notch1 and Notch2. PLoS One. 2009 Aug 24;4(8):e6613. doi: 10.1371/journal.pone.0006613. PMID: 19701457; PMCID: PMC2726630.

Gordon WR, Arnett KL, Blacklow SC. The molecular logic of Notch signaling--a structural and biochemical perspective. J Cell Sci. 2008 Oct 1;121(Pt 19):3109-19. doi: 10.1242/jcs.035683. PMID: 18799787; PMCID: PMC2696053.

Li K, Li Y, Wu W, Gordon WR, Chang DW, Lu M, Scoggin S, Fu T, Vien L, Histen G, Zheng J, Martin-Hollister R, Duensing T, Singh S, Blacklow SC, Yao Z, Aster JC, Zhou BB. Modulation of Notch signaling by antibodies specific for the extracellular negative regulatory region of NOTCH3. J Biol Chem. 2008 Mar 21;283(12):8046-54. doi: 10.1074/jbc.M800170200. Epub 2008 Jan 8. PMID: 18182388.

Estrada K, Fisher C, Blacklow SC. Unfolding of the RAP-D3 helical bundle facilitates dissociation of RAP-receptor complexes. Biochemistry. 2008 Feb 12;47(6):1532-9. doi: 10.1021/bi702076y. Epub 2008 Jan 5. PMID: 18177055.

Del Bianco C, Aster JC, Blacklow SC. Mutational and energetic studies of Notch 1 transcription complexes. J Mol Biol. 2008 Feb 8;376(1):131-40. doi: 10.1016/j.jmb.2007.11.061. Epub 2007 Nov 28. PMID: 18155729; PMCID: PMC2766816.

Aster JC, Pear WS, Blacklow SC. Notch signaling in leukemia. Annu Rev Pathol. 2008;3:587-613. doi: 10.1146/annurev.pathmechdis.3.121806.154300. PMID: 18039126; PMCID: PMC5934586.

Blacklow SC. Versatility in ligand recognition by LDL receptor family proteins: advances and frontiers. Curr Opin Struct Biol. 2007 Aug;17(4):419-26. doi: 10.1016/j.sbi.2007.08.017. Epub 2007 Sep 17. PMID: 17870468; PMCID: PMC2766800.

Fang TC, Yashiro-Ohtani Y, Del Bianco C, Knoblock DM, Blacklow SC, Pear WS. Notch directly regulates Gata3 expression during T helper 2 cell differentiation. Immunity. 2007 Jul;27(1):100-10. doi: 10.1016/j.immuni.2007.04.018. Epub 2007 Jul 19. PMID: 17658278; PMCID: PMC2801546.

Koduri V, Blacklow SC. Requirement for natively unstructured regions of mesoderm development candidate 2 in promoting low-density lipoprotein receptor-related protein 6 maturation. Biochemistry. 2007 Jun 5;46(22):6570-7. doi: 10.1021/bi700049g. Epub 2007 May 9. PMID: 17488095.

Gordon WR, Vardar-Ulu D, Histen G, Sanchez-Irizarry C, Aster JC, Blacklow SC. Structural basis for autoinhibition of Notch. Nat Struct Mol Biol. 2007 Apr;14(4):295-300. doi: 10.1038/nsmb1227. Epub 2007 Apr 1. Erratum in: Nat Struct Mol Biol. 2007 May;14(5):455. PMID: 17401372.

Nam Y, Sliz P, Pear WS, Aster JC, Blacklow SC. Cooperative assembly of higher-order Notch complexes functions as a switch to induce transcription. Proc Natl Acad Sci U S A. 2007 Feb 13;104(7):2103-8. doi: 10.1073/pnas.0611092104. Epub 2007 Feb 6. PMID: 17284587; PMCID: PMC1892977.

Weng AP, Millholland JM, Yashiro-Ohtani Y, Arcangeli ML, Lau A, Wai C, Del Bianco C, Rodriguez CG, Sai H, Tobias J, Li Y, Wolfe MS, Shachaf C, Felsher D, Blacklow SC, Pear WS, Aster JC. c-Myc is an important direct target of Notch1 in T-cell acute lymphoblastic leukemia/lymphoma. Genes Dev. 2006 Aug 1;20(15):2096-109. doi: 10.1101/gad.1450406. Epub 2006 Jul 17. Erratum in: Genes Dev. 2007 Mar 1;21(5):625. PMID: 16847353; PMCID: PMC1536060.

Chiang MY, Xu ML, Histen G, Shestova O, Roy M, Nam Y, Blacklow SC, Sacks DB, Pear WS, Aster JC. Identification of a conserved negative regulatory sequence that influences the leukemogenic activity of NOTCH1. Mol Cell Biol. 2006 Aug;26(16):6261-71. doi: 10.1128/MCB.02478-05. PMID: 16880534; PMCID: PMC1592797.

Lee D, Walsh JD, Mikhailenko I, Yu P, Migliorini M, Wu Y, Krueger S, Curtis JE, Harris B, Lockett S, Blacklow SC, Strickland DK, Wang YX. RAP uses a histidine switch to regulate its interaction with LRP in the ER and Golgi. Mol Cell. 2006 May 5;22(3):423-30. doi: 10.1016/j.molcel.2006.04.011. PMID: 16678114.

Fisher C, Beglova N, Blacklow SC. Structure of an LDLR-RAP complex reveals a general mode for ligand recognition by lipoprotein receptors. Mol Cell. 2006 Apr 21;22(2):277-83. doi: 10.1016/j.molcel.2006.02.021. PMID: 16630895.

Nam Y, Sliz P, Song L, Aster JC, Blacklow SC. Structural basis for cooperativity in recruitment of MAML coactivators to Notch transcription complexes. Cell. 2006 Mar 10;124(5):973-83. doi: 10.1016/j.cell.2005.12.037. PMID: 16530044.

Malecki MJ, Sanchez-Irizarry C, Mitchell JL, Histen G, Xu ML, Aster JC, Blacklow SC. Leukemia-associated mutations within the NOTCH1 heterodimerization domain fall into at least two distinct mechanistic classes. Mol Cell Biol. 2006 Jun;26(12):4642-51. doi: 10.1128/MCB.01655-05. PMID: 16738328; PMCID: PMC1489116.

Sanchez-Irizarry C, Carpenter AC, Weng AP, Pear WS, Aster JC, Blacklow SC. Notch subunit heterodimerization and prevention of ligand-independent proteolytic activation depend, respectively, on a novel domain and the LNR repeats. Mol Cell Biol. 2004 Nov;24(21):9265-73. doi: 10.1128/MCB.24.21.9265-9273.2004. PMID: 15485896; PMCID: PMC522238.

Weng AP, Ferrando AA, Lee W, Morris JP 4th, Silverman LB, Sanchez-Irizarry C, Blacklow SC, Look AT, Aster JC. Activating mutations of NOTCH1 in human T cell acute lymphoblastic leukemia. Science. 2004 Oct 8;306(5694):269-71. doi: 10.1126/science.1102160. PMID: 15472075.

NMR Structure of a Prototype LNR Module from Human Notch1.
Vardar D, North CL, Sanchez-Irizarry C, Aster JC, and Blacklow SC. Biochemistry 2003; 42:7061-7067.

Nam Y, Weng AP, Aster JC, Blacklow SC. Structural requirements for assembly of the CSL.intracellular Notch1.Mastermind-like 1 transcriptional activation complex. J Biol Chem. 2003 Jun 6;278(23):21232-9. doi: 10.1074/jbc.M301567200. Epub 2003 Mar 18. PMID: 12644465.

Weng AP, Nam Y, Wolfe MS, Pear WS, Griffin JD, Blacklow SC, Aster JC. Growth suppression of pre-T acute lymphoblastic leukemia cells by inhibition of notch signaling. Mol Cell Biol. 2003 Jan;23(2):655-64. doi: 10.1128/MCB.23.2.655-664.2003. PMID: 12509463; PMCID: PMC151540.

Nam Y, Aster JC, Blacklow SC. Notch signaling as a therapeutic target. Curr Opin Chem Biol. 2002 Aug;6(4):501-9. doi: 10.1016/s1367-5931(02)00346-0. PMID: 12133727.

Wu L, Aster JC, Blacklow SC, Lake R, Artavanis-Tsakonas S, Griffin JD. MAML1, a human homologue of Drosophila mastermind, is a transcriptional co-activator for NOTCH receptors. Nat Genet. 2000 Dec;26(4):484-9. doi: 10.1038/82644. PMID: 11101851.

Rand MD, Grimm LM, Artavanis-Tsakonas S, Patriub V, Blacklow SC, Sklar J, Aster JC. Calcium depletion dissociates and activates heterodimeric notch receptors. Mol Cell Biol. 2000 Mar;20(5):1825-35. doi: 10.1128/MCB.20.5.1825-1835.2000. PMID: 10669757; PMCID: PMC85363.

Aster JC, Simms WB, Zavala-Ruiz Z, Patriub V, North CL, Blacklow SC. The folding and structural integrity of the first LIN-12 module of human Notch1 are calcium-dependent. Biochemistry. 1999 Apr 13;38(15):4736-42. doi: 10.1021/bi982713o. PMID: 10200161.

Tan Z, Forster AC, Blacklow SC, Cornish VW. Amino acid backbone specificity of the Escherichia coli translation machinery. J Am Chem Soc. 2004 Oct 13;126(40):12752-3. doi: 10.1021/ja0472174. PMID: 15469251.

Forster AC, Cornish VW, Blacklow SC. Pure translation display. Anal Biochem. 2004 Oct 15;333(2):358-64. doi: 10.1016/j.ab.2004.06.028. PMID: 15450813.

Forster AC, Tan Z, Nalam MN, Lin H, Qu H, Cornish VW, Blacklow SC. Programming peptidomimetic syntheses by translating genetic codes designed de novo. Proc Natl Acad Sci U S A. 2003 May 27;100(11):6353-7. doi: 10.1073/pnas.1132122100. Epub 2003 May 16. PMID: 12754376; PMCID: PMC164450.

Forster AC, Weissbach H, Blacklow SC. A simplified reconstitution of mRNA-directed peptide synthesis: activity of the epsilon enhancer and an unnatural amino acid. Anal Biochem. 2001 Oct 1;297(1):60-70. doi: 10.1006/abio.2001.5329. PMID: 11567528.

Beglova N, Blacklow SC, Takagi J, Springer TA. Cysteine-rich module structure reveals a fulcrum for integrin rearrangement upon activation. Nat Struct Biol. 2002 Apr;9(4):282-7. doi: 10.1038/nsb779. PMID: 11896403.

Takagi J, Beglova N, Yalamanchili P, Blacklow SC, Springer TA. Definition of EGF-like, closely interacting modules that bear activation epitopes in integrin beta subunits. Proc Natl Acad Sci U S A. 2001 Sep 25;98(20):11175-80. doi: 10.1073/pnas.201420198. PMID: 11572973; PMCID: PMC58703.

Stone JR, Maki JL, Blacklow SC, Collins T. The SCAN domain of ZNF174 is a dimer. J Biol Chem. 2002 Feb 15;277(7):5448-52. doi: 10.1074/jbc.M109815200. Epub 2001 Dec 11. PMID: 11741982; PMCID: PMC1201017.

Zhang Z, Komives EA, Sugio S, Blacklow SC, Narayana N, Xuong NH, Stock AM, Petsko GA, Ringe D. The role of water in the catalytic efficiency of triosephosphate isomerase. Biochemistry. 1999 Apr 6;38(14):4389-97. doi: 10.1021/bi9826759. PMID: 10194358.

Williams AJ, Blacklow SC, Collins T. The zinc finger-associated SCAN box is a conserved oligomerization domain. Mol Cell Biol. 1999 Dec;19(12):8526-35. doi: 10.1128/MCB.19.12.8526. PMID: 10567577; PMCID: PMC84969.