Publications

X. Xu, S. H. Choi, T. Hu, K. Tiyanont, R. Habets, A. J. Groot, M. Vooijs, J. C. Aster, R. Chopra, C. Fryer, and S. C. Blacklow, “Insights into Autoregulation of Notch3 from Structural and Functional Studies of Its Negative Regulatory Region.,” Structure (London, England : 1993), vol. 23, no. 7, pp. 1227–1235, Jul. 2015.

W. R. Gordon, B. Zimmerman, L. He, L. J. Miles, J. Huang, K. Tiyanont, D. G. McArthur, J. C. Aster, N. Perrimon, J. J. Loparo, and S. C. Blacklow, “Mechanical Allostery: Evidence for a Force Requirement in the Proteolytic Activation of Notch.,” Dev Cell, vol. 33, no. 6, pp. 729–736, Jun. 2015.

R. A. J. Habets, A. J. Groot, S. Yahyanejad, K. Tiyanont, S. C. Blacklow, and M. Vooijs, “Human NOTCH2 Is Resistant to Ligand-independent Activation by Metalloprotease Adam17.,” J Biol Chem, vol. 290, no. 23, pp. 14705–14716, Jun. 2015.

B. J. McMillan, B. Schnute, N. Ohlenhard, B. Zimmerman, L. Miles, N. Beglova, T. Klein, and S. C. Blacklow, “A tail of two sites: a bipartite mechanism for recognition of notch ligands by mind bomb e3 ligases.,” Mol Cell, vol. 57, no. 5, pp. 912–924, Mar. 2015.

Y. Yashiro-Ohtani, H. Wang, C. Zang, K. L. Arnett, W. Bailis, Y. Ho, B. Knoechel, C. Lanauze, L. Louis, K. S. Forsyth, S. Chen, Y. Chung, J. Schug, G. A. Blobel, S. A. Liebhaber, B. E. Bernstein, S. C. Blacklow, X. S. Liu, J. C. Aster, and W. S. Pear, “Long-range enhancer activity determines Myc sensitivity to Notch inhibitors in T cell leukemia.,” Proceedings of the National Academy of Sciences, vol. 111, no. 46, pp. E4946–53, Nov. 2014.

M. R. McKeown, D. L. Shaw, H. Fu, S. Liu, X. Xu, J. J. Marineau, Y. Huang, X. Zhang, D. L. Buckley, A. Kadam, Z. Zhang, S. C. Blacklow, J. Qi, W. Zhang, and J. E. Bradner, “Biased multicomponent reactions to develop novel bromodomain inhibitors.,” J Med Chem, vol. 57, no. 21, pp. 9019–9027, Nov. 2014.

K. L. Arnett and S. C. Blacklow, “Analyzing the nuclear complexes of Notch signaling by electrophoretic mobility shift assay.,” Methods Mol Biol, vol. 1187, pp. 231–245, 2014.

H. Wang, C. Zang, L. Taing, K. L. Arnett, Y. J. Wong, W. S. Pear, S. C. Blacklow, X. S. Liu, and J. C. Aster, “NOTCH1-RBPJ complexes drive target gene expression through dynamic interactions with superenhancers.,” Proceedings of the National Academy of Sciences, vol. 111, no. 2, pp. 705–710, Jan. 2014.

S. C. Blacklow, “Refining a Jagged edge.,” Structure (London, England : 1993), vol. 21, no. 12, pp. 2100–2101, Dec. 2013.

K. Tiyanont, T. E. Wales, C. W. Siebel, J. R. Engen, and S. C. Blacklow, “Insights into Notch3 Activation and Inhibition Mediated by Antibodies Directed against Its Negative Regulatory Region.,” J Mol Biol, vol. 425, no. 17, pp. 3192–3204, Sep. 2013.

M. B. Andrawes, X. Xu, H. Liu, S. B. Ficarro, J. A. Marto, J. C. Aster, and S. C. Blacklow, “Intrinsic Selectivity of Notch 1 for Delta-like 4 Over Delta-like 1.,” J Biol Chem, vol. 288, no. 35, pp. 25477–25489, Aug. 2013.

G. Roti, A. Carlton, K. N. Ross, M. Markstein, K. Pajcini, A. H. Su, N. Perrimon, W. S. Pear, A. L. Kung, S. C. Blacklow, J. C. Aster, and K. Stegmaier, “Complementary genomic screens identify SERCA as a therapeutic target in NOTCH1 mutated cancer.,” Cancer Cell, vol. 23, no. 3, pp. 390–405, Mar. 2013.

J. C. Aster and S. C. Blacklow, “Targeting the Notch pathway: twists and turns on the road to rational therapeutics.,” J Clin Oncol, vol. 30, no. 19, pp. 2418–2420, Jul. 2012.

D. J. O'Donovan, I. Stokes-Rees, Y. Nam, S. C. Blacklow, G. F. Schröder, A. T. Brunger, and P. Sliz, “A grid-enabled web service for low-resolution crystal structure refinement.,” Acta Crystallogr D Biol Crystallogr, vol. 68, no. 3, pp. 261–267, Mar. 2012.

S. H. Choi, T. E. Wales, Y. Nam, D. J. O'Donovan, P. Sliz, J. R. Engen, and S. C. Blacklow, “Conformational locking upon cooperative assembly of notch transcription complexes.,” Structure (London, England : 1993), vol. 20, no. 2, pp. 340–349, Feb. 2012.

K. Germar, M. Dose, T. Konstantinou, J. Zhang, H. Wang, C. Lobry, K. L. Arnett, S. C. Blacklow, I. Aifantis, J. C. Aster, and F. Gounari, “T-cell factor 1 is a gatekeeper for T-cell specification in response to Notch signaling.,” Proceedings of the National Academy of Sciences, Nov. 2011.

N. J. Wang, Z. Sanborn, K. L. Arnett, L. J. Bayston, W. Liao, C. M. Proby, I. M. Leigh, E. A. Collisson, P. B. Gordon, L. Jakkula, S. Pennypacker, Y. Zou, M. Sharma, J. P. North, S. S. Vemula, T. M. Mauro, I. M. Neuhaus, P. E. Leboit, J. S. Hur, K. Park, N. Huh, P.-Y. Kwok, S. T. Arron, P. P. Massion, A. E. Bale, D. Haussler, J. E. Cleaver, J. W. Gray, P. T. Spellman, A. P. South, J. C. Aster, S. C. Blacklow, and R. J. Cho, “Loss-of-function mutations in Notch receptors in cutaneous and lung squamous cell carcinoma.,” Proceedings of the National Academy of Sciences, vol. 108, no. 43, pp. 17761–17766, Oct. 2011.

B. Zhao, J. Zou, H. Wang, E. Johannsen, C.-W. Peng, J. Quackenbush, J. C. Mar, C. C. Morton, M. L. Freedman, S. C. Blacklow, J. C. Aster, B. E. Bernstein, and E. Kieff, “Epstein-Barr virus exploits intrinsic B-lymphocyte transcription programs to achieve immortal cell growth.,” Proceedings of the National Academy of Sciences, vol. 108, no. 36, pp. 14902–14907, Sep. 2011.

H. Wang, J. Zou, B. Zhao, E. Johannsen, T. Ashworth, H. Wong, W. S. Pear, J. Schug, S. C. Blacklow, K. L. Arnett, B. E. Bernstein, E. Kieff, and J. C. Aster, “Genome-wide analysis reveals conserved and divergent features of Notch1/RBPJ binding in human and murine T-lymphoblastic leukemia cells.,” Proceedings of the National Academy of Sciences, Jul. 2011.

M. A. Calderwood, S. Lee, A. M. Holthaus, S. C. Blacklow, E. Kieff, and E. Johannsen, “Epstein-Barr virus nuclear protein 3C binds to the N-terminal (NTD) and beta trefoil domains (BTD) of RBP/CSL; only the NTD interaction is essential for lymphoblastoid cell growth.,” Virology, vol. 414, no. 1, pp. 19–25, May 2011.

K. Tiyanont, T. E. Wales, M. Aste-Amezaga, J. C. Aster, J. R. Engen, and S. C. Blacklow, “Evidence for increased exposure of the Notch1 metalloprotease cleavage site upon conversion to an activated conformation.,” Structure (London, England : 1993), vol. 19, no. 4, pp. 546–554, Apr. 2011.

J. C. Aster, S. C. Blacklow, and W. S. Pear, “Notch signalling in T-cell lymphoblastic leukaemia/lymphoma and other haematological malignancies.,” J. Pathol., vol. 223, no. 2, pp. 262–273, Jan. 2011.

J. C. Aster, N. Bodnar, L. Xu, F. Karnell, J. M. Milholland, I. Maillard, G. Histen, Y. Nam, S. C. Blacklow, and W. S. Pear, “Notch ankyrin repeat domain variation influences leukemogenesis and myc transactivation.,” PLoS ONE, vol. 6, no. 10, p. e25645, 2011.

T. D. Ashworth, W. S. Pear, M. Y. Chiang, S. C. Blacklow, J. Mastio, L. Xu, M. Kelliher, P. Kastner, S. Chan, and J. C. Aster, “Deletion-based mechanisms of Notch1 activation in T-ALL: key roles for RAG recombinase and a conserved internal translational start site in Notch1.,” Blood, vol. 116, no. 25, pp. 5455–5464, Dec. 2010.

K. Keeshan, W. Bailis, P. H. Dedhia, M. E. Vega, O. Shestova, L. Xu, K. Toscano, S. N. Uljon, S. C. Blacklow, and W. S. Pear, “Transformation by Tribbles homolog 2 (Trib2) requires both the Trib2 kinase domain and COP1 binding.,” Blood, vol. 116, no. 23, pp. 4948–4957, Dec. 2010.

H. Liu, A. W. S. Chi, K. L. Arnett, M. Y. Chiang, L. Xu, O. Shestova, H. Wang, Y.-M. Li, A. Bhandoola, J. C. Aster, S. C. Blacklow, and W. S. Pear, “Notch dimerization is required for leukemogenesis and T-cell development.,” Genes Dev, vol. 24, no. 21, pp. 2395–2407, Nov. 2010.

K. L. Arnett, M. Hass, D. G. McArthur, M. X. G. Ilagan, J. C. Aster, R. Kopan, and S. C. Blacklow, “Structural and mechanistic insights into cooperative assembly of dimeric Notch transcription complexes.,” Nat Struct Mol Biol, vol. 17, no. 11, pp. 1312–1317, Nov. 2010.

P. H. Dedhia, K. Keeshan, S. Uljon, L. Xu, M. E. Vega, O. Shestova, M. Zaks-Zilberman, C. Romany, S. C. Blacklow, and W. S. Pear, “Differential ability of Tribbles family members to promote degradation of C/EBPalpha and induce acute myelogenous leukemia.,” Blood, vol. 116, no. 8, pp. 1321–1328, Aug. 2010.

R. A. Kovall and S. C. Blacklow, “Mechanistic insights into Notch receptor signaling from structural and biochemical studies.,” Curr Top Dev Biol, vol. 92, pp. 31–71, 2010.

M. Aste-Amezaga, N. Zhang, J. E. Lineberger, B. A. Arnold, T. J. Toner, M. Gu, L. Huang, S. Vitelli, K. T. Vo, P. Haytko, J. Z. Zhao, F. Baleydier, S. L'Heureux, H. Wang, W. R. Gordon, E. Thoryk, M. B. Andrawes, K. Tiyanont, K. Stegmaier, G. Roti, K. N. Ross, L. L. Franlin, H. Wang, F. Wang, M. Chastain, A. J. Bett, L. P. Audoly, J. C. Aster, S. C. Blacklow, and H. E. Huber, “Characterization of Notch1 antibodies that inhibit signaling of both normal and mutated Notch1 receptors.,” PLoS ONE, vol. 5, no. 2, p. e9094, 2010.

C. Del Bianco, A. Vedenko, S. H. Choi, M. F. Berger, L. Shokri, M. L. Bulyk, and S. C. Blacklow, “Notch and MAML-1 complexation do not detectably alter the DNA binding specificity of the transcription factor CSL.,” PLoS ONE, vol. 5, no. 11, p. e15034, 2010

R. E. Moellering, M. Cornejo, T. N. Davis, C. Del Bianco, J. C. Aster, S. C. Blacklow, A. L. Kung, D. G. Gilliland, G. L. Verdine, and J. E. Bradner, “Direct inhibition of the NOTCH transcription factor complex.,” Nature, vol. 462, no. 7270, pp. 182–188, Nov. 2009.

Y. Yashiro-Ohtani, Y. He, T. Ohtani, M. E. Jones, O. Shestova, L. Xu, T. C. Fang, M. Y. Chiang, A. M. Intlekofer, S. C. Blacklow, Y. Zhuang, and W. S. Pear, “Pre-TCR signaling inactivates Notch1 transcription by antagonizing E2A.,” Genes Dev, vol. 23, no. 14, pp. 1665–1676, Jul. 2009.

W. R. Gordon, M. Roy, D. Vardar-Ulu, M. Garfinkel, M. R. Mansour, J. C. Aster, and S. C. Blacklow, “Structure of the Notch1-negative regulatory region: implications for normal activation and pathogenic signaling in T-ALL.,” Blood, vol. 113, no. 18, pp. 4381–4390, Apr. 2009.

W. R. Gordon, D. Vardar-Ulu, S. L'Heureux, T. Ashworth, M. J. Malecki, C. Sanchez-Irizarry, D. G. McArthur, G. Histen, J. L. Mitchell, J. C. Aster, and S. C. Blacklow, “Effects of S1 cleavage on the structure, surface export, and signaling activity of human Notch1 and Notch2.,” PLoS ONE, vol. 4, no. 8, p. e6613, 2009.

W. R. Gordon, K. L. Arnett, and S. C. Blacklow, “The molecular logic of Notch signaling--a structural and biochemical perspective.,” J Cell Sci, vol. 121, no. 19, pp. 3109–3119, Oct. 2008.

K. Li, Y. Li, W. Wu, W. R. Gordon, D. W. Chang, M. Lu, S. Scoggin, T. Fu, L. Vien, G. Histen, J. Zheng, R. Martin-Hollister, T. Duensing, S. Singh, S. C. Blacklow, Z. Yao, J. C. Aster, and B.-B. S. Zhou, “Modulation of Notch signaling by antibodies specific for the extracellular negative regulatory region of NOTCH3.,” J Biol Chem, vol. 283, no. 12, pp. 8046–8054, Mar. 2008.

K. Estrada, C. Fisher, and S. C. Blacklow, “Unfolding of the RAP-D3 helical bundle facilitates dissociation of RAP-receptor complexes.,” Biochemistry, vol. 47, no. 6, pp. 1532–1539, Feb. 2008.

C. Del Bianco, J. C. Aster, and S. C. Blacklow, “Mutational and energetic studies of Notch 1 transcription complexes.,” J Mol Biol, vol. 376, no. 1, pp. 131–140, Feb. 2008.

J. C. Aster, W. S. Pear, and S. C. Blacklow, “Notch signaling in leukemia.,” Annu Rev Pathol, vol. 3, pp. 587–613, 2008.

S. C. Blacklow, “Versatility in ligand recognition by LDL receptor family proteins: advances and frontiers.,” Curr Opin Struct Biol, vol. 17, no. 4, pp. 419–426, Aug. 2007.

T. C. Fang, Y. Yashiro-Ohtani, C. Del Bianco, D. M. Knoblock, S. C. Blacklow, and W. S. Pear, “Notch directly regulates Gata3 expression during T helper 2 cell differentiation.,” Immunity, vol. 27, no. 1, pp. 100–110, Jul. 2007

V. Koduri and S. C. Blacklow, “Requirement for natively unstructured regions of mesoderm development candidate 2 in promoting low-density lipoprotein receptor-related protein 6 maturation.,” Biochemistry, vol. 46, no. 22, pp. 6570–6577, Jun. 2007.

W. R. Gordon, D. Vardar-Ulu, G. Histen, C. Sanchez-Irizarry, J. C. Aster, and S. C. Blacklow, “Structural basis for autoinhibition of Notch.,” Nat Struct Mol Biol, vol. 14, no. 4, pp. 295–300, Apr. 2007.

Y. Nam, P. Sliz, W. S. Pear, J. C. Aster, and S. C. Blacklow, “Cooperative assembly of higher-order Notch complexes functions as a switch to induce transcription.,” Proc Natl Acad Sci USA, vol. 104, no. 7, pp. 2103–2108, Feb. 2007.

A. P. Weng, J. M. Millholland, Y. Yashiro-Ohtani, M. L. Arcangeli, A. Lau, C. Wai, C. Del Bianco, C. G. Rodriguez, H. Sai, J. Tobias, Y. Li, M. S. Wolfe, C. Shachaf, D. Felsher, S. C. Blacklow, W. S. Pear, and J. C. Aster, “c-Myc is an important direct target of Notch1 in T-cell acute lymphoblastic leukemia/lymphoma.,” Genes Dev, vol. 20, no. 15, pp. 2096–2109, Aug. 2006.

M. Y. Chiang, M. L. Xu, G. Histen, O. Shestova, M. Roy, Y. Nam, S. C. Blacklow, D. B. Sacks, W. S. Pear, and J. C. Aster, “Identification of a conserved negative regulatory sequence that influences the leukemogenic activity of NOTCH1.,” Mol Cell Biol, vol. 26, no. 16, pp. 6261–6271, Aug. 2006.

D. Lee, J. D. Walsh, I. Mikhailenko, P. Yu, M. Migliorini, Y. Wu, S. Krueger, J. E. Curtis, B. Harris, S. Lockett, S. C. Blacklow, D. K. Strickland, and Y.-X. Wang, “RAP uses a histidine switch to regulate its interaction with LRP in the ER and Golgi.,” Mol Cell, vol. 22, no. 3, pp. 423–430, May 2006.

C. Fisher, N. Beglova, and S. C. Blacklow, “Structure of an LDLR-RAP complex reveals a general mode for ligand recognition by lipoprotein receptors.,” Mol Cell, vol. 22, no. 2, pp. 277–283, Apr. 2006.

Y. Nam, P. Sliz, L. Song, J. C. Aster, and S. C. Blacklow, “Structural basis for cooperativity in recruitment of MAML coactivators to Notch transcription complexes.,” Cell, vol. 124, no. 5, pp. 973–983, Mar. 2006.

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M. J. Malecki, C. Sanchez-Irizarry, J. L. Mitchell, G. Histen, M. L. Xu, J. C. Aster, and S. C. Blacklow, “Leukemia-associated mutations within the NOTCH1 heterodimerization domain fall into at least two distinct mechanistic classes.,” Mol Cell Biol, vol. 26, no. 12, pp. 4642–4651, Jun. 2006.

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C. Sanchez-Irizarry, A. C. Carpenter, A. P. Weng, W. S. Pear, J. C. Aster, and S. C. Blacklow, “Notch Subunit Heterodimerization and Prevention of Ligand-Independent Proteolytic Activation Depend, Respectively, on a Novel Domain and the LNR Repeats,” Mol Cell Biol, vol. 24, no. 21, pp. 9265–9273, Oct. 2004.

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Activating mutations of NOTCH1 in human T cell acute lymphoblastic leukemia.
Weng AP, Ferrando AA, Lee W, Morris JP, Silverman LB, Sanchez-Irizarry C, Blacklow SC, Look T, and Aster JC. Science. 2004 Oct 8;306(5694):269-71.

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NMR Structure of a Prototype LNR Module from Human Notch1.
Vardar D, North CL, Sanchez-Irizarry C, Aster JC, and Blacklow SC. Biochemistry 2003; 42:7061-7067.

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S.C. Structural Requirements for Assembly of the CSL/Intracellular Notch1/Mastermind-like 1 Transcriptional Activation Complex.
Nam Y, Weng AP, Aster JC and Blacklow, J. Biol. Chem. 2003, 278:21232-21239.

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Growth Suppression of Pre-T Acute Lymphoblastic Leukemia Cells by Inhibition of Notch Signaling.
Weng AP, Nam Y, Wolfe MS, Pear WS, Griffin JD, Blacklow SC, and Aster JC. Mol Cell Biol 2003; 23:655-664.

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Notch signaling as a therapeutic target.
Nam Y, Aster JC, Blacklow SC. Curr Opin Chem Biol. 2002 Aug;6(4):501-9. Review.

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MAM1, a human homologue of Drosophila Mastermind, is a transcriptional co-activator for notch receptors.
Wu L, Aster JC, Blacklow SC, Lake R, Artavanis-Tsakonas S, and Griffin JD. Nature Genetics 2000;26:484-489.

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Calcium depletion dissociates and activates heterodimeric notch receptors.
Rand MD, Grimm LM, Artavanis-Tsakonas S, Patriub V, Blacklow SC, Sklar J, Aster JC. Mol Cell Biol. 2000 Mar;20(5):1825-35

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Folding and structural integrity of the first LIN12 module of human Notch1 are calcium dependent.
Aster JC, Simms WB, Zavala-Ruiz Z, Patriub V, North CL, and Blacklow SC. Biochemistry 1999;38:4736-42.

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Amino Acid Backbone Specificity of the Escherichia coli Translation Machinery.
Tan Z, Forster AC, Blacklow SC, Cornish VW.J Am Chem Soc. 2004;126:12752-12753.

Pure Translation Display.
Forster, AC, Cornish, V, Blacklow, SC. Analytical Biochemistry 2004; 333(2):358-64.

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Programming peptidomimetic syntheses by translating genetic codes designed de novo.
Forster, A., Tan, Z., Nalam, M.N., Lin, H., Qu, H., Cornish, V.W., and Blacklow, S.C. Proc Natl Acad Sci U S A. 2003; 100(11):6353-6357.

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A Simplified Reconstitution of mRNA-Directed Peptide Synthesis: Activity of the Epsilon Enhancer Sequence and an Unnatural Amino Acid.
Forster, A, Weissbach, H, and Blacklow, SC. Analytical Biochemistry 2001; 297: 60-70.

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Cysteine-Rich Module Structure Reveals a Fulcrum for Integrin Rearrangement Upon Activation.
Beglova, N, Blacklow, SC, Takagi, J, Springer, TA. Nature Structure Biology 2002; 9:282-287.

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Definition of EGF-like, closely interacting modules that bear activation epitopes in integrin beta subunits.
Takagi, J, Beglova, N, Yalamanchili, P, Blacklow, SC, and Springer, TA. PNAS 2001; 98:11175-11180.

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The SCAN domain of ZNF174 is a dimer.
Stone JR, Maki JL, Blacklow SC, and Collins T. J Biol Chem 2002;277:5448-5452.

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The role of water in the catalytic efficiency of triosephosphate isomerase.
Zhang Z, Komives EA, Sugio S, Blacklow SC, Narayana N, Xuong NH, Stock AM, Petsko GA, and Ringe D. Biochemistry 1999;38:4389-97.

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The zinc finger-associated SCAN box is an oligomerization domain.
Williams, AJ, Blacklow, SC, and Collins, T. Mol Cell Biol 1999;19:8526-8535.

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